| Location | Dispatch Channel (s) | Counties Covered |
| Arcadia | Ch-2 | Desoto, Glades, Hardee, Hendry, Highlands |
| Bradenton | Ch-3 | Manatee |
| Brooksville | Ch-3 & 154.860 | Citrus, Hernando, Pasco, Sumter |
| *Cocoa | 800 Mhz Astro trunked | Brevard |
| *DeLand | 800 Mhz Astro trunked | Flagler, Lake, Seminole, Volusia |
| *Ft. Lauderdale | 800 Mhz Astro trunked | Broward |
| Ft. Myers | Ch-1 & 154.845 | Collier, Lee |
| Gainsville | Ch-3 | Alachua, Dixie, Gilchrist, Lafayette, Levy |
| Jacksonville | Ch-1 | Duval, Nassau |
| Lake City | Ch-3 & 154.815 | Baker, Bradford, Columbia,Hamilton,Suwannee, Union |
| Lakeland | Ch-1 | Polk |
| *Lake Worth | 800 Mhz Astro trunked | Entire Turnpike |
| *Lantana | 800 Mhz Astro trunked | Indian River, Martin, Okeechobee, Palm Beach, St. Lucie |
| Madison | Ch-1 | Jefferson, Madison, Taylor |
| *Miami | 800 Mhz Astro trunked | Dade, Monroe |
| Ocala | Ch-1 | Marion |
| *Orlando | 800 Mhz Astro trunked | Orange, Osceola |
| Palatka | Ch-3 | Clay, Putnam, St. Johns Note: Jacksonville dispatches from 10pm-6am |
| Panama City | Ch-1 | Bay, Calhoun, Gulf, Holmes |
| Panama City | Ch-2 | Jackson, Washington |
| Pensacola | Ch-1 | Okaloosa, Walton |
| Pensacola | Ch-3 | Escambia, Santa Rosa |
| Tallahassee | Ch-3 | Leon, Franklin, Gadsen, Liberty, Wakulla |
| Tampa | Ch-1 | Pinellas |
| Tampa | Ch-2 | Hillsborough |
| Venice | Ch-2 | Charlotte, Sarasota |
Notes:
When possible, the channel used for specific counties is noted.
The Turnpike Communications Center in Orlando has closed and was consolidated into a new center in Lake Worth.
This list does not include remote transmitter sites. In some areas a mobile unit may use the name of the closest tower site to their location. i.e. "700 Cross City". This assists the dispatcher in selecting the correct tower.
Most communication centers that have switched to 800 Mhz have removed their old base channels and should be retaining use of Ch-4. VHF mobiles are to be removed later.